metadata: {"signature": "0x920d373edeb312afc33c981669e9e1b11d663f4b4c13b88f3064098030af62982e8b26a6579b2b9ae1e68492dd4bd0ebec826d2c19216d8ac8c8fa591c8ca5de1b", "application": {"round": "0x5eb890e41c8d2cff75ea942085e406bb90016561", "answers": [{"type": "email", "hidden": true, "question": "Email Address", "questionId": 0, "encryptedAnswer": {"ciphertext": "eGaO/LLZ/CzekJtjKADuH+8BOg0hkWcAgdk5NH+59xwiPxagLvicff60hIZM5QyL", "encryptedSymmetricKey": "d498e8f173348d7ae7e0fb9386ff71f1f7e7accb7685fb93f0d9363679978794dc0efe9e118eae58236cee114db93219ff7690120945f2ca9946ec2784df3cd282116d50c1eb5f2017060903a83b352e34a7418ad335e5f15b60936c7eab93ad83f7ef491ac68da854a8b3ffb35268e4b1158d4db9c2f3ecf831f28233bf842b0000000000000020efed8a53409ca279f374c2ca3139ca574fe916993a192f1c817ed896f319a8b8989ee0c8b912f86e9f6f5457e9062c8e"}}, {"type": "short-answer", "hidden": true, "question": "Telegram Handle", "questionId": 1, "encryptedAnswer": {"ciphertext": "+9UD4Qk+IkBUCEiMp4NwHi/ului157pyYm6r2exqiyg=", "encryptedSymmetricKey": "c9c1d74fabf9cab0724701ccb3d3ee52318263d3a5652b0b2d0520110b520c6c46ac57864806b852b99c5262d33b7906c8d0e7d7070af1e3cf31cdc15cc3790d89e1a1ef3bed5127353ca2c46f5d5c97cca0e6886feedd4d25b453f60c50a7aab2594c1649c19f05e893e271982c31aa23593438867b6a49770363dc97e47f6900000000000000209a20d91605bcd7f801f167ca444600141a627f180dcd94e8e739896138c290c7ff2872785380528e93609cd4780181b7"}}, {"type": "short-answer", "hidden": true, "question": "Gitcoin Gov Forum Handle (gov.gitcoin.co)", "questionId": 2, "encryptedAnswer": {"ciphertext": "yQ4YsIwyTRQnKO0I/qZZzT6eC4z/c9w7BnBCnOQ9BzA=", "encryptedSymmetricKey": "0851f517dd9c7588ed9b8c92f578019f9e3bc99e8d9bd72b9a8cf7a07c15f381bf24ad03e4821d3e34f33b743eb1f604bf185e5b9f315e3e9d064a3aa2c85ef5435e3f9871a131f7971636683395dca65999e30f0312a05d59d172a4d5a4d8b89338091f5097a7340e93dd0e163e59108d99a3696afd4f465f4cc750fda992ff0000000000000020fcbc491e2593a3267f2f8aaab0b674f8510b033c15a689c9400a58a57acf004ec6d9417ac0501ebb062091f0247eddb4"}}, {"type": "link", "hidden": false, "question": "Link to your Public Group Chat (Discord or Telegram )", "questionId": 3}, {"type": "number", "answer": "5500", "hidden": false, "question": "Total prior funding for the project in USD", "questionId": 4}, {"type": "paragraph", "answer": "I received money previously to finish building my lab from a gitcoin grant. We are ready to go.", "hidden": false, "question": "Please expand on how much funding you\u2019ve received from funding sources and when? (VC, grants by foundation, token sales, etc)", "questionId": 5}, {"type": "number", "answer": "2", "hidden": false, "question": "Total team size", "questionId": 6}, {"type": "number", "answer": "12", "hidden": false, "question": "How old (in Months) is the project?", "questionId": 7}, {"type": "address", "hidden": true, "question": "If you participated in past grant rounds using a different project payout wallet address, please share it here:", "questionId": 8}, {"type": "paragraph", "hidden": true, "question": "Profiles or socials for other main team members publicly associated with the project. These may be used to determine eligibility of the project.: ", "questionId": 9}, {"type": "multiple-choice", "answer": "Yes", "hidden": true, "question": "Have you read and confirmed that your grant abides by the Program General Eligibility Policy? https://gitcoin.notion.site/GG19-Eligibility-50843c8b3ab44ad297731a00efa6e389?pvs=4", "questionId": 10}, {"type": "paragraph", "answer": "We are developing a probiotic to reduce the greenhouse gas emissions produced by cattle. ", "hidden": false, "question": "Please detail how your project meets the Climate Solutions Round eligibility criteria. https://climate-coordination-network.notion.site/Climate-Solutions-Round-Eligibility-bf14ea78d9d24a2f8952cb5270f11b00?pvs=4", "questionId": 11}, {"type": "paragraph", "answer": "We have shifted our direction from looking to produce genetically modified algae to instead be selectively breeding Methylococcus . This was due to restrictions on GMO's and shortcomings in genetic expression with genes in  spirulina.", "hidden": false, "question": "If you've received funding in past grant rounds, please share any new updates or milestones from the prior months. You can also include the challenges you've faced. ", "questionId": 12}, {"type": "multiple-choice", "answer": "No", "hidden": false, "question": "Have you minted a Hypercert for your project? ", "questionId": 13}, {"type": "link", "hidden": false, "question": "If Yes, please provide a link to your Hypercert (https://hypercerts.org/app/gallery-all) ", "questionId": 14}, {"type": "paragraph", "answer": "Each year, a single cow will emit ~220 pounds of methane.  One ton of methane is = 25 CO2 carbon credits. We are looking to reduce the methane emitted by each cow by >50%", "hidden": false, "question": "How do you measure the impact of your project? Please provide examples and/or standard metrics. This might be used in future impact reports ", "questionId": 15}, {"type": "paragraph", "answer": "We have shifted our direction from looking to produce genetically modified algae to instead be selectively breeding Methylococcus .", "hidden": false, "question": "Anything else you'd like to share about your project, previous work, or other project affiliations? Anything you'd like to add that may help in determining project eligibility?", "questionId": 16}, {"type": "multiple-choice", "answer": "Yes", "hidden": true, "question": "Would you like to opt-in to future messages from the Climate Coordination Network (CCN) for updates, information, and/or interviews? ", "questionId": 17}, {"type": "multiple-choice", "answer": "Yes", "hidden": true, "question": "Would you like to opt-in to us sharing your information with select third-parties for investment opportunities? ", "questionId": 18}, {"type": "multiple-choice", "answer": "Natural Based Solutions", "hidden": false, "question": "Which of these categories best represents your project? Choose from: ", "questionId": 19}, {"type": "checkbox", "answer": ["North America"], "hidden": false, "question": "Location/Region where the project is based: Choose from: ", "questionId": 20}, {"type": "checkbox", "answer": ["North America"], "hidden": false, "question": "Primary Location/Region where the project is making an impact: Choose from: ", "questionId": 21}, {"type": "multiple-choice", "answer": "Yes", "hidden": false, "question": "Would you like to be included in an additional 150k sub-round funded by Shell? All grantees must agree to a KYC process to be eligible to receive these funds. ", "questionId": 22}], "project": {"id": "43114:0xDF9BF58Aa1A1B73F0e214d79C652a7dd37a6074e:5", "title": "Next Generation Probiotics for Green Agriculture. ", "metaPtr": {"pointer": "bafkreicdfcqca4cpeedl6rtgudl3yuphfr5we6onv4jyxlgblo2wtw37ia", "protocol": "1"}, "website": "https://www.defibiolabs.com", "createdAt": 1699408569284, "credentials": {}, "description": " Domesticate and develop  probiotics to reduces greenhouse gas emissions and improves livestock health.  \n\n\nTitle: Directed Evolution of Methylococcus capsulatus as a Probiotic for Reducing Methane Emissions in Livestock\nIntroduction\nMethane emissions from livestock, particularly from enteric fermentation in cattle, contribute significantly to global greenhouse gas emissions. Implementing strategies to reduce methane production in livestock has the potential to mitigate climate change while maintaining agricultural productivity. This proposal aims to use directed evolution to engineer Methylococcus capsulatus, a methanotrophic bacterium, to act as a probiotic in cattle and livestock that can reduce methane emissions.\nObjective\nTo develop a novel Methylococcus capsulatus strain with enhanced methane consumption capabilities and the ability to survive and thrive in the gastrointestinal tract of cattle and livestock. This will be achieved by:\nEngineering Methylococcus capsulatus to optimize methane oxidation.\nEnhancing the bacterium's resistance to the gastrointestinal environment in cattle and livestock.\nEvaluating the effectiveness of the engineered Methylococcus capsulatus as a probiotic in reducing methane emissions.\nMethods\nDirected Evolution of Methylococcus capsulatus\n\n\nExpected Outcomes and Impact\nThe successful development of an engineered Methylococcus capsulatus strain with improved methane oxidation capabilities and resistance to gastrointestinal conditions has the potential to significantly reduce methane emissions from cattle and livestock. This would contribute to climate change mitigation efforts and promote sustainable livestock farming practices.\nMoreover, the use of Methylococcus capsulatus as a probiotic may have additional benefits, such as improving feed efficiency and animal health, which would enhance the overall sustainability and profitability of the livestock industry.\nIn conclusion, this proposal outlines a novel approach to reducing methane emissions from livestock using directed evolution to engineer Methylococcus capsulatus as a probiotic. If successful, this strategy could make a significant impact on global greenhouse gas emissions and support sustainable agriculture.\n\nAt Defi Biolabs, our mission is to revolutionize the livestock industry by harnessing the power of next generation probiotics to reduce greenhouse gas emissions and improve the wellbeing of livestock. We are dedicated to creating innovative, sustainable, and eco-friendly solutions that benefit both the environment and the global agricultural community. Through cutting-edge research and techbio we strive to make a lasting, positive impact on the world by fostering responsible and sustainable livestock management practices for a greener, healthier earth.\n", "lastUpdated": 0, "projectTwitter": "Defibiolabs"}, "recipient": "0x632Fd6D5E53A8661e751a964c9d7c9aa63Ecaa5A"}}